Journal: Life Science Alliance

Article Title: Deletion of exon 2 in ALS-linked Sptlc1 causes lethality in homozygous mice but not in heterozygotes

doi: 10.26508/lsa.202503605

Figure Lengend Snippet: (A) GFAP immunofluorescence staining in 18-mo-old lumbar spinal cord region. Average GFAP fluorescence intensity quantification is on right (n = 4 mice per group). (B) qRT–PCR confirms no significant difference in Gfap levels (n = 7 mice per group). (C) IBA1 immunofluorescence staining in 18-mo-old lumbar spinal cord region. Average IBA1 fluorescence intensity quantification is on right (n = 4 mice per group). (D) qRT–PCR confirms no significant difference in Iba1 levels (n = 7 mice per group). (E) NeuN was used as a neuronal marker, and NeuN-positive cell quantification is on right. DAPI was used to stain nuclei. N = 4 mice per group. Data are presented as the mean ± SEM, t test, significance threshold set at P < 0.05.

Article Snippet: The following primary antibodies were used: anti-NeuN antibody (guinea pig polyclonal, 266004, 1:1,000; Synaptic Systems), anti-IBA1 antibody (rabbit polyclonal, 01919741, 1:1,000; Wako), and anti-GFAP antibody (rabbit polyclonal, ab7260, 1:500; Abcam).

Techniques: Immunofluorescence, Staining, Fluorescence, Quantitative RT-PCR, Marker

Journal: Life Science Alliance

Article Title: Deletion of exon 2 in ALS-linked Sptlc1 causes lethality in homozygous mice but not in heterozygotes

doi: 10.26508/lsa.202503605

Figure Lengend Snippet: (A) GFAP and IBA1 immunofluorescence staining in 18-mo-old brain sagittal section. The GFAP and IBA1 signals in Sptlc1 ΔE2/ + were compared with WT littermates. Higher magnification images are shown. NeuN was used as a neuronal marker. DAPI was used to stain nuclei. N = 4 mice per group. (B) Average fluorescence intensity quantifications of GFAP and IBA1, as well as the number of NeuN-positive cells. (C) qRT–PCR confirms no significant difference in Gfap or Iba1 levels in the cortex region of Sptlc1 ΔE2/ + compared with controls or Sptlc +/+ 18-mo-old animals ( P < 0.05, t test, n = 7 mice per group).

Article Snippet: The following primary antibodies were used: anti-NeuN antibody (guinea pig polyclonal, 266004, 1:1,000; Synaptic Systems), anti-IBA1 antibody (rabbit polyclonal, 01919741, 1:1,000; Wako), and anti-GFAP antibody (rabbit polyclonal, ab7260, 1:500; Abcam).

Techniques: Immunofluorescence, Staining, Marker, Fluorescence, Quantitative RT-PCR

Journal: Cell reports

Article Title: Impaired spatial coding and neuronal hyperactivity in the medial entorhinal cortex of aged APP knock-in mice

doi: 10.1016/j.celrep.2026.117505

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Guinea pig polyclonal anti-NeuN (1:5,000) , Synaptic Systems , Cat#266 004; RRID: AB_2619988.

Techniques: Recombinant, Staining, Knock-In, Control, Software, Imaging, Fluorescence, Microscopy